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ISEH Networking Story: Membership Pays Off

Posted By Connections Editor, Wednesday, July 1, 2015
Updated: Monday, June 29, 2015

Patricia Ernst, an ISEH member, recently traveled to Germany and with the aid of many fellow ISEH members was able to complete some important work in Germany thanks to her ISEH Connections.

Do you have a friend or colleague that could benefit from ISEH membership. Refer them today!

 

 

By: Patricia Ernst, ISEH Board of Directors 

 

Recently we came across a major question in our work that we thought could be answered using a unique mouse model generated by a lab in Germany, headed by Prof. A. Francis Stewart. Although I was a fan of the lab’s published work, I did not really know anyone there. My PhD student, Yufei Chen, felt that this animal model might be critical for her thesis work, yet when we considered what it would take to import the animals, it seemed impractical to request the strain, go through quarantine, back-cross, intercross, etc. So we decided to ask Francis if we could come to his lab in Germany, work with the strain, generate some transformed cell lines and send them back the U.S. for further characterization. He welcomed us, but some of the techniques we planned were not routinely performed by their group, so access to the proper TC hoods, reagents, Miltenyi magnets, etc. might not be easy. Bringing an entire suitcase of reagents also did not seem practical. So on a whim, I checked the ISEH membership to see if anyone I knew in the field was located in Dresden, and I found Claudia Waslkow, who I also did not know but had seen at ISEH meetings. So again on a whim, I emailed her and asked if we might be able to use her equipment to do the experiment – luckily she was thoroughly welcoming about the idea and in fact had a collaboration ongoing with Francis’ lab. Since Yufei’s visa status made it tough for her to go to Europe, I decided to do it myself and started making the preparations. Members of Claudia’s institute were instrumental in helping me throw together a travel and lodging plan, which was tough for me since there are a number of German holidays in May that I did not know about, and since I do not speak or read German, some hotel web sites were Greek to me. An administrator in Claudia’s institute (Julia Seifert) found a nice family run hotel within walking distance that was affordable and kindly set up the reservation for me. 

 

Then in the planning process I started to become aware that it might not be so simple to just mail ahead all the reagents that I needed for the experiment. Here, two other ISEH members were instrumental in helping me make sure this worked out smoothly. Hartmut Geiger (ISEH member) had a lot of experience shipping things back and forth to Germany and even staff in his lab in Cincinnati (Kalpana Nattamai) helped me tremendously with how precisely to label and package biological materials to ensure that there were no delays in the shipping process. The remaining challenge was shipping viral supernatants-I was very worried that if they got hung up in customs, the entire trip would fall apart. So Mick Milsom (ISEH member), already located within Germany, was kind enough to send to Dresden some of his precious viral supernatant so I could ensure everything was in place when I got there. This worked beautifully, and with some help ordering via the unfamiliar European offices of Stem Cell Technology and Miltenyi, I got everything in place just barely as I arrived in Dresden. Aside from some Lufthansa problems, I managed to get there, find my way into the lab and get set up with all the shipped materials, find the mice from Francis’ lab and borrow whatever else was needed from Claudia’s lab. Once there, Kristin Arndt, a postdoc in Claudia’s lab helped me tremendously to my way in an unfamiliar building, get all the reagents together and show me where the relevant equipment was. I managed to squeeze in a seminar, as well as quick tourism with Claudia on the weekend! Despite running out of Euros, having my ATM card turned off, and having my departure reservation “disappeared” from the Lufthansa system, I managed to get back home where then Kristin and Julia shipped on dry ice some of the materials I generated while in Claudia’s lab. Later, Kerstin Schmidt, a PhD student working with Francis and Konstantinos Anastassiadis, confirmed the genotypes after I was back in the U.S. and sent me the data so I could feel sure that in the fog of jetlag I did not mix up samples.

 

There were a lot of challenges and barriers to setting up this experiment but the amazing thing is that for each challenge, help from a scientist overcame those barriers. I could not have set up this elaborate plan without the structure of ISEH to look up like-minded hematologists by region and could not have pulled off the whole experiment without significant effort from ISEH scientists. From borrowing everything in Claudia’s lab, to help from students and postdocs getting in and out of the institute when I needed, to help from Mick and Hartmut to find the best ways to get materials to Germany, there were so many people who were critical in this plan. I wanted to share this story with ISEH members, particularly trainees, because it illustrates how the “ISEH family” helps each other even without ongoing collaborations or tangible reasons to be helpful. ISEH scientists just share a motivation to get experiments accomplished! I think ISEH selects for this type of individual and that is why I found it so easy to reach out and ask to borrow things and camp out in people’s labs halfway across the globe. One just cannot put a value on this type of networking-even though I may never get the chance to truly repay all the people who helped me, I feel confident that I can “give back” in a way through the ISEH network through who knows what kind of future activities!

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Defining the map back to stemness

Posted By Connections Editor, Friday, May 1, 2015
Updated: Wednesday, April 29, 2015

By: Eirini Trompouki and Teresa V. Bowman 

 

Thousands of bone marrow transplantations take place each year in US alone.
Although broadly employed, the use of transplantation is hampered by the lack of appropriate donor cells. Even when there is a matched donor, the number of cells is often insufficient, thus hampering the full utility of the therapy. There is clearly an immediate need to enhance our ability to create or expand hematopoietic stem cells (HSCs). To this end, George Daley and Derrick Rossi, both from Harvard Medical School, presented their approaches, for overcoming this challenge at the most recent ISEH webinar entitled “Hematopoietic specification from pluripotent cell lines vs. reprogramming of somatic cells” (now available for viewing online at http://www.iseh.org/?ISEHWebinars).

Reprogramming of differentiated cells to progenitor cell types holds great promise for medical applications. However, reprogramming to HSCs has proven particularly challenging. The Daley and Rossi groups as well as others including the webinar moderator, Kateri Moore and her group from Mt. Sinai Hospital, have devised approaches to reprogram various cell types back to a multipotent state. Dr. Daley’s group is striving to impart long-term multilineage potential to embryonically derived human hematopoietic progenitors. His lab has established a protocol to direct human embryonic stem cells or induced pluripotent stem cells to a primitive myeloid progenitor. Using this population as starting material and following a Yamanaka-like strategy to reprogramming, Daley’s group determined that five factors (HOXA9, RORA, ERG, SOX4, and MYB) were sufficient to promote multipotent engraftment potential (Doulatov et al. Cell Stem Cell 2013). They have now expanded the transcription factor repertoire to include NFIA and DACH2, which are needed to convey better lymphoid potential, a population that is usually under-represented in most blood-forming efforts. Finally, Dr. Daley also showed that these methods can be successfully used to model human disease. He presented an unpublished model of a hereditary blood disorder, Diamond-Blackfan Anemia, demonstrating that many facets of the disease can be recapitulated both in vitro and in vivo. He ended with a short vignette on a chemical screen that uncovered autophagy as a novel therapeutic target for suppressing the erythroid defects in DBA.

Dr. Rossi discussed his lab’s approach to reprogram mature progenitor and effector blood cells back to multilineage long-term HSCs using a murine model. After intense screening, the Rossi group defined a set of six factors (Hlf, Pbx1, Prdm5, Runx1t1/Eto, Lmo2, and Zfp37) that are needed to reprogram either common myeloid progenitors of pre/pro-B cells into induced hematopoietic stem cells (iHSC) (Riddell et al. 2014). After further technical refinement, they also demonstrated that inclusion of Meis1 and Mycn and importantly the use of polycistronic vectors enhanced reprogramming. Thus, specific factors but also their respective expression levels can severely affect reprogramming. Having successfully employed the powers of an in vivo system, Dr. Rossi also eluded to on-going work in his laboratory to improve ex vivo culture conditions for HSC expansion using small molecule screening. The hope is to establish reprogramming conditions that can then be used to generate iHSCs ex vivo.

During the webinar it became clear that there is a lack of commonality among the transcription factors identified by the Daley and Rossi groups as well as others such as Kateri Moore or Shahin Rafii (Weill Cornell). Do factors identified in murine systems play a conserved role in humans? How important is the initial cell that is used for reprogramming? The investigators agree that the starting cell type and epigenetic state influence or even determine the identity of factors needed to reprogram a given cell type. From the discussion, it was well understood that it is much easier to reprogram closely related blood cells than other differentiated cell types like fibroblasts. The multiple players in the HSC reprogramming field now have a wide choice of potential factors to try to reprogram their favorite cell type. The investigators agreed the more the merrier when it comes to labs contributing to the field of HSC reprogramming. Working together to solve the problem will only improve the system and advance science faster. Throughout the webinar, it was gratifying to hear not only interesting data but also the collaborative nature of the work and the openness of the investigators to share their reagents. With this pace and attitude, there is a high possibility that deriving patient-specific HSCs is not science fiction but a proximal reality.

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Register now for the upcoming ISEH webinar

Posted By Connections Editor , Friday, May 1, 2015
Updated: Thursday, April 30, 2015

Clonal Evolution of Pre-Leukemic HSCs in AML 

Wednesday, 3 June 2015, 2:30 - 4:00 pm EST 

 

The identity of the cell of origin for hematologic malignancy as well as the mechanism via which cellular transformation is achieved, have both been topics of much debate within the hematology community for many years. The advent of next generation sequencing technologies has provided a route through which increased resolution of this process can be attained. Using this technology, a number of recent high profile publications have characterized an age-associated increase in the genomic mutation frequency within the hematopoietic stem cell compartment associated with non-malignant expansion of individual stem cell clones. Frequently, these mutations occur in epigenetic modifiers such as DNMT3a, IDH1/2 and TET2, which are also often found in leukemia. This suggests that epigentic instability may be a major driving force in this clonal evolution and that this process may be an age-related step towards transformation.

In the next ISEH webinar, two leaders in this field, Drs. Ross Levine and Ravi Majeti, will discuss the recent advances in this research topic and will discuss the insights that their work brings to our understanding of HSC clonal evolution during aging and its relationship to leukemogenesis. Dr. Ross Levine is a physician scientist at Memorial Sloan Kettering Cancer Center, New York, who leads a research group that has a major focus on understanding the genetic basis of myeloid malignancies. Dr. Ravi Majeti is also a clinician scientist who leads a research group based at Stanford School of Medicine with research interests centred on the characterization and targeting of leukemia stem cells.

The webinar will take place on Wednesday, 3 June 2015 at 2:30 to 4:00 pm EST and will be moderated by Dr. Ann Mullally, Assistant Professor of Medicine at Harvard Medical School and Brigham and Women’s Hospital, Boston. Be sure to register to see what promises to be a great webinar on one of the current hot topics in hematology led by world-renowned experts in the field. This will be followed by an interactive discussion where you can submit questions to any of the webinar participants.

 

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Society News: August/September 2014

Posted By Connections Editor, Friday, September 5, 2014
Updated: Wednesday, September 3, 2014

ISEH announces board of directors election results for 2014

ISEH closed its 2014 board of director’s election 16 July. In this year's election 133 members voted, and the results were extremely close, speaking to the high quality of candidates on the ballot.

Your newly elected ISEH Board Members include: 
                                              
Vice President: Timm Schroeder                                        Director, America: Emery Bresnick

 

 

                                       
Director, Europe: Hartmut Geiger                                        Director, Pacific Rim: Sarah Ellis



The new leadership began their terms on 24 August 2014, the final day of the ISEH 43rd Annual Scientific Meeting.

ISEH thanks its outgoing board members:

• Past President: Elaine Dzierzak
• Director: James Palis
• Director: Marella de Bruijn
• Director: Bertie Göttgens

 

They each committed their time and expertise to guiding and directing ISEH towards future success over the last three or more years.

 

Visit the new ISEH Career Center Today

View a posting, or request a quote to post a job/fellowship at your own facility. In the past month alone, ISEH.org had over 22,000 page views in the month of August alone, a posting on ISEH.org is a great way to get your open position in front of the some of the world’s premier scientists and the rising stars in the field. 

Current career and fellowship opportunities: 

Dartmouth College/University of Colorado Denver - Our group is seeking an individual for a postdoctoral fellowship who has experience in gene regulation mechanisms or hematopoietic stem cell biology to join our projects to clarify how epigenetic regulators coordinate self-renewal and differentiation programs in normal stem cells and in leukemia. Please send a letter of intent, contact information for 3 references, and cv to Patricia Ernst at patricia.ernst@dartmouth.edu. 


Indiana University - Post-Doctoral Positions Available in Area of Hematopoietic and Induced Pluripotent Stem Cells. Dr. Broxmeyer is seeking motivated and productive postdoctoral candidates with a PhD, MD, or MD/PhD, in the area of regulation of hematopoietic stem and progenitor cells, and induced pluripotent stem cells. Priority will be given to those candidates with prior experience and publications in these areas. Please send a CV and the names of three references to: Hal E. Broxmeyer, PhD, Distinguished Professor, Department of Microbiology and Immunology, IU School of Medicine, 635 Barnhill Drive, MS 420, Indianapolis, IN 46202 or to hbroxmey@iupui.edu. Indiana University is an EEO/AA employer, M/F/D/V

 

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Call for Nominations & New Member Forum

Posted By Connections Editor , Thursday, May 1, 2014

Leadership is Calling!

ISEH –International Society of Experimental Hematology is looking for members to serve on the Board of Directors.  We urge you to take a few minutes of your time to nominate candidates you feel have the skills and interest to manage and guide the Society on your behalf. Or, if you have a passion and are committed to serving ISEH, you can nominate yourself.

Help ensure that the Nominating Committee presents a balanced slate of candidates for election later in the year.

 Open Positions:
Vice President (4 year term)
4 Directors (3 year term)

All nominations received by Monday, 19 May (12:00 am CST) will be forwarded to the Nominating Committee to finalize the ballot. All ISEH members who are nominated will be considered for candidacy. Please note that former presidents, honorary, emeritus, and associate members, as well current office holders are not eligible for nomination.
For your convenience you can click here to view the current Board of Directors.




New ISEH Members Only Resource: ISEH Ask the Expert Forum

Ask the Experts forum is a member only benefit - featuring two experts from the field of hematology. This month's forum features David Kent (University of Cambridge) and Brad Dykstra (Harvard Medical School). Both will be available for the next month to answer and discuss questions from ISEH members. 

Join the Discussion! Carolina Abramovich, Managing Editor at Experimental Hematology asked: 

Single cell analysis - Is it worth the effort? In last Tuesday's ISEH webinar entitled
 "Heterogeneity in normal and malignant HSC: peas in a pod or apples and oranges?", both Dr. De Haan and Dr. Dick showed very interesting data from single cell analysis. Could you please elaborate on the benefits of single cell experiments.  Are they worth the effort? What extra information do they give?"


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New task force on a mission to generate membership

Posted By Connections Editor, Wednesday, January 8, 2014
The new Membership & Marketing Task Force, headed up by Hanna Mikkola and David Traver, has some big plans to help grow the ISEH membership in 2014. 

The new task force, with its truly international contingency, is beginning a campaign to contact lapsed members from years past, and personally reaching out to other colleagues in the field to encourage new membership and involvement with our growing organization. 

With the kickoff of the 2014 renewal season this past October, ISEH is seeing some of the highest renewal numbers in recent years. The introduction of the new 2-year membership option has also generated some excitement among our membership. Now it’s time for us to spread the word about ISEH and encourage our friends and colleagues to get involved by renewing their memberships or joining for the first time. 

What can you do to help? Tell us why you’re an ISEH member.

Was it a mentor who told you about ISEH? Did you first join to attend a meeting? What makes you renew year after year? ISEH would like to know. We’d like to show our potential new members why our membership is so dedicated to ISEH. Email your thoughts and stories about ISEH to mdaniels@iseh.org by 30 January, 2014.

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2013 webinar series concludes with lecture on xenograft models

Posted By Connections Editor, Tuesday, January 7, 2014

On December 16th 2013, ISEH had the privilege to host the webinar of Dr. Gwenn Danet-Desnoyers entitled "Xenograft models of human hematopoiesis and leukemia". Dr. Danet-Desnoyers is the Director of the Stem Cell and Xenograft Core and an adjunct Associate Professor at theUniversity of PennsylvaniaSchool of Medicine. He has help organized one of the most comprehensive resource lab in the US with a large tissue bank and a variety of xenograft models to accelerate the treatment of leukemic patients.

During the first part of the talk, Dr. Danet-Desnoyers highlighted the requirements for the xenogenic reconstitution of the human hemato-lymphoid system and explained the importance of human cytokines to provide signals that support human cell survival, development and function in mice. He presented studies from his lab that: 1) compared the ability of CD34+ cells from cord blood, fetal liver and BLT to reconstitution in NSG mice; 2) compared human immune reconstitution in NSG, NRG and NSG-SGM3 mice, and 3) investigated the effect of exogenous support with IL-15/IL-15Ra complex on NK development. Dr. Danet-Desnoyers concluded this part of the talk with a discussion of the limitations of the established models and possible solutions.

The second part of the webinar was devoted to patient-derived xenograft models for AML. Dr. Danet-Desnoyers presented data showing that AML cells that do not engraft in NSG mice are capable of engrafting NSG-SGM3 mice, while conserving AML cell surface markers in primary and secondary recipients. He then followed with the question of whether leukemic stem cells are restricted to the CD34+ CD38- phenotype and clearly showed that CD38+ cells can repopulate NSG mice.

The webinar concluded with answers to a number of questions from the audience read by Dr. Martin Carroll, who acted as moderator.

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Stem cell niche webinar recap

Posted By Connections Editor, Friday, November 1, 2013
Updated: Monday, October 28, 2013

Contributed by Eugenia Figueroa-Flores, ISEH New Investigator Committee

Technology is changing the way we do and share science. As an international society, webinars are a useful tool to keep our members informed and connected throughout the year.

On September 23rd, the New Investigator Committee hosted the second ISEH webinar, featuring a discussion between two of our prominent members and leaders in the field of the hematopoietic stem cell niche. David Scadden and Paul Frenette gave an overview of the field and shared their latest results. With Andreas Trumpp as the moderator, the webinar/debate flowed smoothly and was interesting from beginning to end.

The speakers demonstrated how improved technologies have reshaped our view of the stem cell niche and given us a clearer picture of the murine bone marrow architecture and the location of hematopoietic stem cells.

Mesenchymal stromal cells were the big winners from our event, as both speakers agreed on their relevance as key regulators of the stem cell niche. The hematopoietic stem cell niche field is under constant refinement. Whether the endosteal or vascular niche is the main site for hematopoiesis remains unanswered. Paul Frenette showed detailed analysis of sinusoid distribution on the bone marrow that supports a perivascular location for hematopoietic stem cells, although David Scadden pointed out that under conditions unfavorable for the vasculature, osteoblasts could take on the supportive role. As with most biology, context is key.


If you missed the live broadcast or want to view it again, we invite you to check our webpage and follow our social media, as the webinar recording will be available soon. We were inspired by our speakers and from the 100% satisfaction of our participants, so were you! We invite you to stay tuned for the next webinar from ISEH and from the New Investigator Committee coming soon.

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Enhancements to ISEH login and membership renewal

Posted By ISEH Headquarters, Friday, November 1, 2013
Updated: Monday, October 28, 2013
ISEH Membership renewal season is upon us once again, and it’s never been easier to renew you membership online.

Your ISEH.org login information has changed!

New Login: your email address, example: ISEHmember@yourfacility.com
New Password*: welcome1

Once you have logged in, pay your dues by clicking on "Securely renew your membership"

Also new for 2014, renew for two-years and then cross "renew ISEH membership” off your to-do list for next year!

*Do you know 5+ scientists who should be members of ISEH and have been waiting for the right moment? The time has come, ISEH is offering group membership rates** for groups of five or more new members from the same facility, email info@iseh.org for more details.

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ISEH Finance Committee Seeks Volunteer

Posted By ISEH Headquarters, Monday, September 9, 2013
Updated: Friday, June 28, 2013

ISEH Finance Committee Seeks Volunteer

Objective:

ISEH is undergoing an RFP process to find a financial investment partner. The ISEH Finance Committee is seeking an ISEH member to assist in conducting approximately 5 interviews of potential investment firms. Due to the geographic composition of the committee an individual within North America who is familiar with the United States financial system is needed.

Criteria for Volunteer:

o The project will start immediately with an end goal date of November 1

o This project should take no more than 10 hours of committed time

o A general understanding of the US financial system and basic knowledge on investing needed.

o The position will be directed by and report to the ISEH Finance Committee

o The final recommendation made through this position will be presented to the ISEH Board of Directors for approval

If you are interested in this position, please contact Kim Eskew at keskew@iseh.org or +1.312.673.4963.

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8/23/2018 » 8/26/2018
ISEH 47th Annual Meeting - Los Angeles, CA

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