On December 16th 2013, ISEH
had the privilege to host the webinar of Dr. Gwenn
Danet-Desnoyers entitled "Xenograft models of human
hematopoiesis and leukemia". Dr.
Danet-Desnoyers is the Director of the Stem Cell and Xenograft Core and
an adjunct Associate Professor at theUniversity of
PennsylvaniaSchool of Medicine. He has help organized one of the most
comprehensive resource lab in the US with a large tissue bank and a variety of
xenograft models to accelerate the treatment of leukemic patients.
the first part of the talk, Dr. Danet-Desnoyers highlighted the requirements for the xenogenic reconstitution of the
human hemato-lymphoid system and explained the importance of human
cytokines to provide signals that support human cell
survival, development and function in mice. He
presented studies from his lab that: 1) compared the ability of CD34+ cells from cord blood, fetal liver and
BLT to reconstitution in NSG mice; 2) compared human immune reconstitution in NSG,
NRG and NSG-SGM3 mice, and 3) investigated the effect of exogenous support with
IL-15/IL-15Ra complex on NK development.
Dr. Danet-Desnoyers concluded this part of the talk
with a discussion of the limitations of the established models and possible
part of the webinar was devoted to patient-derived xenograft models for AML.
presented data showing that AML cells that do not engraft in NSG mice are
capable of engrafting NSG-SGM3 mice, while conserving AML cell surface markers in
primary and secondary recipients. He then followed with the question of whether
leukemic stem cells are restricted to the CD34+ CD38- phenotype
and clearly showed that CD38+ cells can repopulate NSG mice.
The webinar concluded with answers to a number
of questions from the audience read by Dr. Martin
Carroll, who acted as