4th 2013-"Combinatorial Transcriptional Control of Blood Stem and Progenitor Cells"
Dr. Bertie Göttgens from the Cambridge Institute for Medical
Research presented his group's research efforts into constructing hematopoietic
transcriptional networks from single cell data. The webinar highlighted the
application of the Fluidigm BioMark platform, a basically a high throughput qPCR system
that allows the assay of multiple genes in multiple cells in parallel, and
principal component analysis (PAC), to study such networks. Dr. Göttgens exemplified how this approach combined
with ChIP-Seq, transgenic mice and
repressor assays, demonstrated a negative correlation between Gata2 and Gfi1 which was
present only in stem cells. The last part of the webinar was dedicated to a
live demonstration of the use of gene set control analysis for the
identification of transcription networks using the HAEMCODE, a comprehensive
collection of ChIP-Sep experiments on the hematopoietic development.